| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Adult somatic progenitor cells and hematopoiesis in oysters
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| Autores: |
Jemaa, Mohamed
Morin, Nathalie
Cavelier, Patricia
Cau, Julien
Strub, Jean Marc
Delsert, Claude
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| Data: |
2014-09
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| Ano: |
2014
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| Palavras-chave: |
Hematopoiesis
Adult somatic progenitor cells
Hemocytes
Mollusk
Marine invertebrates
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| Resumo: |
Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further evidenced by phosphorylated-histone H3 mitotic staining. Finally, these small cells most abundant in the IFS epithelium were found positive for the stemness marker Sox2. We provide evidence for hematopoiesis by showing that co-expression of Sox2 and Cu/Zn SOD, a hemocyte-specific enzyme, does not occur in the gill epithelial cells but rather in the underlying tissues and vessels. We further confirm the hematopoietic features of these cells by the detection of Filamin, a protein specific for a sub-population of hemocytes, in large BrdU-labeled cells bathing into gill vessels. Altogether, our data show that progenitor cells differentiate into hemocytes in gill, which suggests that hematopoiesis occurs in oyster gills.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00197/30802/29172.pdf
DOI:10.1242/jeb.106575
http://archimer.ifremer.fr/doc/00197/30802/
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| Editor: |
Company Of Biologists Ltd
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-09 , Vol. 217 , N. 17 , P. 3067-3077
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| Direitos: |
2013. Published by The Company of Biologists Ltd
info:eu-repo/semantics/openAccess
restricted use
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